ULTRASTRUCTURAL STUDY OF HUMAN SPERM ACROSOME DURING ACETIC ACID EXTRACTION OF ACROSIN
نویسندگان
چکیده
منابع مشابه
Acrosin accelerates the dispersal of sperm acrosomal proteins during acrosome reaction.
Using homologous recombination, we have previously produced male mice carrying a disruptive mutation (Acr-/-) in the acrosin gene. Although Acr-/- mouse sperm lacking the acrosin protease activity still penetrated the zona pellucida and fertilized the egg, the mutant sperm exhibited a delay in penetration of the zona pellucida solely at the early stages after insemination. To further elucidate ...
متن کاملAcrosin activation follows its surface exposure and precedes membrane fusion in human sperm acrosome reaction.
Evidence has accumulated suggesting multiple roles of acrosin in fertilization, including its participation in early steps of gamete recognition and binding. However, the implication of acrosin in many of these processes is not compatible with its presumptive sequestration within the sperm acrosome until a late phase of the acrosome reaction. In an earlier study (J. Tesarik, J. Drahorad, J. Pek...
متن کاملThe histochemical localization of acrosin in guinea-pig sperm after the acrosome reaction.
The protease acrosin is widely considered to be an essential component of a zona lysin which enables sperm to penetrate the zona pellucida of the egg. Sperm form a characteristic penetration slit little wider than the sperm head itself and this has long suggested that any zona lysin is attached to the sperm surface after an acrosome reaction. This paper provides the first ultrastructural eviden...
متن کاملImmunodetection of acrosin during the acrosome reaction of hamster, guinea-pig and human spermatozoa.
Mammalian sperm acrosomes contain a trypsin-like protease called acrosin which causes limited and specific hydrolysis of the extracellular matrix of the mammalian egg, the zona pellucida. Acrosin was localized on hamster, guinea-pig and human sperm using monoclonal and polyclonal antibodies to human acrosin labelled with colloidal gold. This was visualized directly with transmission electron mi...
متن کاملAssessment of released acrosin activity as a measurement of the sperm acrosome reaction.
AIM To develop a method for assessing sperm function by measuring released acrosin activity during the acrosome reaction (AR). METHODS Human semen samples were obtained from 24 healthy donors with proven fertility after 3-7 days of sexual abstinence. After collection, samples were liquefied for 30 min at room temperature. Standard semen parameters were evaluated according to World Health Orga...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1975
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0420385